F4

Anti cancer Protocol Prohibits tumor cell migration F4 peptide CNPEDCLYPVSHAHQR derived from collagen XIX, inhibits melanoma cell migration in vitro and cancer progression in a mouse melanoma model. Studies confirmed the anti angiogenic properties of F4 peptide and demonstrated that F4 peptide inhibited VEGF induced pseudo tube formation on Matrigel by endothelial cells and endothelial sprouting in a rat aortic ring assay. By affinity chromatography, we identified αvβ3 and α5β1 integrins as potential receptors for F4 peptide on endothelial cell surface. Solid phase assays proved the direct interaction between F4 and both integrins. Taken together, the results demonstrate that F4 peptide is a potent antitumor agent inhibiting both angiogenesis and tumor cell migration.